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| Reyes-Montes,María del Rocío; Frías-De-León,María Guadalupe; Victoriano-Pastelín,Isai; Acosta-Altamirano,Gustavo; Duarte-Escalante,Esperanza. |
| ABSTRACT At present, there is no standardized marker that is routinely used in clinical laboratories to diagnose coccidioidomycosis. Thus, the goals of this study were to obtain a sequence characterized amplified region (SCAR) marker for the identification of Coccidioides spp., evaluate its specificity and sensitivity in fungal DNA-spiked blood and sputum samples, and compare it with previously described molecular markers. Specific amplified fragment length polymorphism (AFLP) amplicons for Coccidioides immitis and Coccidioides posadasii were cloned into the vector pGEM® -T Easy vector and sequenced to develop a SCAR marker. Oligonucleotides were designed to identify Coccidioides spp. by polymerase chain reaction (PCR), and the specificity and sensitivity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; SCAR; Coccidioidomycosis; Coccidioides spp.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500322 |
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| Sahaza,Jorge H.; Rodríguez-Arellanez,Gabriela; Canteros,Cristina E.; Reyes-Montes,María del Rocío; Taylor,Maria Lucia. |
| ABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Latin America; H. capsulatum; Yeasts; Thermotolerance; Thermosensitivity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044 |
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